Research FacilitiesBiomolecular Mass Spectrometry and Proteomics

The biological mass spectrometry and proteomics laboratory is equipped with two state of the art LTQ-Orbitrap mass spectrometers. To extract the maximum amount of information from a sample the laboratory operates in a highly cooperative and interactive fashion. We provide unbiased protein identification and detection of post translational modifications using multiple fragmentation techniques such as CID, ETD and HCD aided by a variety of proteases. Furthermore, we support a range of workflows for protein quantification such as SILAC, 15N labeling and itraq. Separations such as SDS-PAGE and reversed phase, ion exchange, HILIC and affinity liquid chromatography are used to maximise protein coverage. The use of bioinformatic approaches is crucial to the success of many projects and in addition to standard approaches for protein identification and quantification such as Mascot and Maxquant we contribute to the statistical interpretation of the data. We are also experienced in advanced mass spectrometry methods for the analysis of nucleic acids, metabolites and intact proteins.