Research FacilitiesFlow Cytometry

The analysis and physical isolation of cells according to their phenotypic and functional properties is an essential technique in cell biology. By measuring the fluorescence emitted by individual cells labelled with fluorescent probes, specific sub populations of cells can be characterized and isolated for further investigation.

A broad spectrum of fluorescent probes are available, including antibodies specific for cell surface or intracellular molecules, dyes to determine the cellular content of DNA or other bio-molecules, and indicators of biophysical properties such as membrane potential or intracellular second messengers.

The facility provides state of the art instrumentation including two bench-top fluorescence analysers (BD FACSCalibur and BD LSR II) and three fluorescence-activated cell sorters (BD FACSVanatage SE, BD FACSAria IIu and BD Influx) suitable for high-speed, multiparametric sorting (up to 25,000 cells per second), with capability of up to fourteen fluorescence parameters. Continually expanding, the diverse array of laser and filter configurations available enable this facility to be capable of most current flow cytometry protocols.

Flow Cytometry
Facility head

James Elliott (Dr)

Flow Cytometrist
Telephone 38330
Email
Facility staff

Magdalena Opanowicz (Dr)

Telephone 38330
Email
Admin contact

Tathiana Santana (Ms)

Telephone 38236
Email
Academic advisor

Matthias Merkenschlager (Professor)

Telephone 38239
Email
Contact details
Telephone: +44 (0) 20 8383 8330/8291
Facsimile: +44 (0) 20 8383 8338
email: magdalena.opanowicz@csc.mrc.ac.uk
james.elliott@csc.mrc.ac.uk

Flow Cytometry bookings page
Imperial College, London
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