Research Groups > Lymphocyte Development

“We use lymphocytes as models to study how gene expression patterns are transmitted through cell division, and to explore the molecular basis of lineage choice”

We study transcriptional and epigenetic mechanisms that underlie cellular differentiation and experimental reprogramming. Our core research activities include the following interrelated areas:
  • Pluripotency and reprogramming
  • Polycomb repressor complexes in stem cells
  • Cohesin function in gene expression and genome organisation
  • Ikaros family transcription factors
We combine classical cell biology and genetics approaches with current technologies to address the events involved in maintaining embryonic stem (ES) cell pluripotency versus differentiation towards mesoderm, endoderm and ectoderm, as well as the mechanisms of T and B cell lineage choice and differentiation.

DNA synthesis (green) in B cells undergoing pluripotent reprogramming.
DNA synthesis (green) in B cells undergoing pluripotent reprogramming. Click to enlarge.











  Lymphocyte Development
Group heads

Amanda Fisher (Professor)

Telephone 38249 (Director) 38238/38242 (Group)
Email

Matthias Merkenschlager (Professor)

Telephone 38239
Email
Group members

Hakan Bagci (Mr)

Telephone 32145
Email

Kotryna Bloznelyte (Ms)

Telephone 32145
Email

Karen Brown (Dr)

Telephone 38286
Email

Ludovica Bruno (Dr)

Telephone 32145
Email

Lesly Calderon Dominguez (Dr)

Telephone 38286
Email

Irene Cantone (Miss)

Telephone 38286
Email

Lee Cooper (Mr)

Telephone 32145
Email

Sergi Cuartero Betriu (Dr)

Telephone 38286
Email

Isabel Ferreiros Vidal (Dr)

Telephone 38286
Email

Amelie Feytout (Dr)

Telephone x38236
Email

Preksha Gupta (Miss)

Telephone 32145
Email

Elizabeth Ing-Simmons (Ms)

Thais Lavagnolli (Ms)

Telephone 32145
Email

Ziwei Liang (Miss)

Telephone 32145
Email

Andy Malinowski (Mr)

Telephone 32145
Email

Allifia Abbas Newsholme (Dr)

Telephone 32145
Email

Jorge Soza Ried (Mr)

Telephone 38286
Email

Stephan Sauer (Dr)

Research Associate
Email

Vlad Seitan (Dr)

Telephone 38286
Email

Matthew Van de Pette (Dr)

Telephone 38286
Email
Admin contact

Tathiana Santana (Ms)

Telephone 38236
Email
Contact details
Telephone: +44 (0) 20 8383 8238/8239
Facsimile: +44 (0) 20 8383 8338
Selected publications
Tsubouchi, T., Soza-Ried, J., Brown, K., Piccolo, F. M., Cantone, I., Landeira, D., Bagci, H., Hochegger, H., Merkenschlager, M., Fisher, A. G., (2013). DNA synthesis is required for reprogramming mediated by stem cell fusion. Cell 152 (4), 873-883. Abstract

Seitan, V. C., Faure, A. J., Zhan, Y., McCord, R. P. P., Lajoie, B. R., Ing-Simmons, E., Lenhard, B., Giorgetti, L., Heard, E., Fisher, A. G., Flicek, P., Dekker, J., Merkenschlager, M., 2013. Cohesin-based chromatin interactions enable regulated gene expression within preexisting architectural compartments. Genome Research 23 (12), 2066-2077. Abstract

Piccolo, F. M., Bagci, H., Brown, K. E., Landeira, D., Soza-Ried, J., Feytout, A., Mooijman, D., Hajkova, P., Leitch, H. G., Tada, T., Kriaucionis, S., Dawlaty, M. M., Jaenisch, R., Merkenschlager, M., Fisher, A. G., (2013). Different roles for tet1 and tet2 proteins in Reprogramming-Mediated erasure of imprints induced by EGC fusion. Molecular Cell 49 (6), 1023-1033.  Abstract

Seitan VC, Hao B, Tachibana-Konwalski K, Lavagnolli T, Mira-Bontenbal H, Brown KE, Teng G, Carroll T, Terry A, Horan K, Marks H, Adams DJ, Schatz DG, Aragon L, Fisher AG, Krangel MS, Nasmyth K, Merkenschlager M, (2011) A role for cohesin in t-cell-receptor rearrangement and thymocyte differentiation. Nature 476, 467–471. Abstract

Jørgensen, H. F. and Fisher, A. G. (2010). Can Controversies be put to REST? Nature 467, E3–E4. Abstract

Pereira, C. F., Piccolo, F. M., Tsubouchi, T., Sauer, S., Ryan, N. K., Bruno, L., Landeira, D., Santos, J., Banito, A., Gil, J. Koseki, H., Merkenschlager, M. and Fisher, A. G. (2010). ESCs require Prc2 to direct the successful reprogramming of differentiated cells toward pluripotency. Cell Stem Cell 6, 547–556. Abstract

Landeira, D., Sauer, S., Poot, R., Dvorkina, M., Mazzarella, L., Jørgensen, H. F., Pereira, C. F., Leleu, M., Piccolo, F. M., Spivakov, M., Brookes, E., Pombo, A., Fisher, C., Skarnes, W. C., Snoek, T., Bezstarosti, K., Demmers, J., Klose, R. J., Casanova, M., Tavares, L., Brockdorff, N., Merkenschlager, M., Fisher, A. G. (2010). Jarid2 is a Prc2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of Prc1 and RNA polymerase II to developmental regulators. Nature Cell Biology 12, 618–624. Abstract

Hadjur, S, Williams, L. M., Ryan, N. K., Cobb, B. S., Sexton, T., Fraser, P., Fisher, A. G., Merkenschlager, M. (2009). Cohesins form chromosomal cis-interactions at the developmentally regulated IFNG locus. Nature 460, 410–413. Abstract

Parelho, V., Hadjur, S., Spivakov, M., Leleu, M., Sauer, S., Gregson, H. C., Jarmuz, A., Canzonetta, C., Webster, Z., Nesterova, T., Cobb, B. S., Yokomori, K., Dillon, N., Aragon, L., Fisher, A. G. & Merkenschlager, M. (2008). Cohesins functionally associate with CTCF on mammalian chromosome arms. Cell 132, 422-433. Abstract

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